Triple-ligation strategy with advantages over directional cloning.
نویسندگان
چکیده
منابع مشابه
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ed here improves upon the concept of directional cloning without forfeiting any of its advantages. The advantages include single-step cloning, high-efficiency generation of the desired recombinant molecule and low background self-ligation of vector. The added benefits of the triple-ligation strategy include easy resolution of vector fragments for gel purification, the ability to invert an inser...
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ورودعنوان ژورنال:
- BioTechniques
دوره 21 4 شماره
صفحات -
تاریخ انتشار 1996